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International Journal of Agriculture and Nutrition

Vol. 6, Issue 1, Part A (2024)

An analysis of DNA extraction and genetic improvement in plant

Author(s):

Gopika MK and Dr. Sunil Chauhan

Abstract:

Several wild species of Limonium are indigenous to the southern parts of Italy, demonstrating the genus's adaptability to the Mediterranean climate and habitat. As a result of its beauty and versatility, Limonium is cultivated all over the globe for use in floral arrangements, both wet and dry. The decorative market is driven in large part by the novelty and aesthetic value of new designs. The research used Limonium sinensis var. Golden Diamond plants that had been regrown from tissue culture. The vegetation was once gathered from K.F. Bio-plants Pvt. Ltd. in Pune-411036, India. Seedlings were nurtured under greenhouse conditions in clay pots (15 cm in diameter) with a mixture of garden soil, sand, and cow manure (1:1:1). Axillary shoot proliferation was induced from explants of immature inflorescence stems, while dormant shoot elongation (DSE) and independent shoot elongation (ISE) were induced from explants of leaves. Severe abiotic stressors, such as those caused by droughts, floods, and other natural catastrophes, as well as developing diseases and a shrinking supply of arable land, are now posing a serious danger to global crop output. Providing enough plant-based goods for a rising global population is undeniably agriculture's primary goal. Growing demand is expected to raise food needs by 70% by 2050, yet the improvements in production and food quality delivered by "The Green Revolution" are not nearly enough to keep up with the pace. In conclusion, through the present study, for the first time, in vitro regenerated plants of Limonium sinensis var. Golden diamond obtained via axillary shoot formation from inflorescence culture, direct or indirect somatic embryogenesis from leaf explants have been genetically analyzed through RAPD and ISSR markers and it was found that regenerants obtained from axillary branching of inflorescence and direct somatic embryogenesis (DSE) from leaf were genetically stable after in vitro propagation whereas regenerants obtained from indirect somatic embryogenesis (ISE) showed polymorphism.

Pages: 52-56  |  177 Views  59 Downloads


International Journal of Agriculture and Nutrition
How to cite this article:
Gopika MK and Dr. Sunil Chauhan. An analysis of DNA extraction and genetic improvement in plant. Int. J. Agric. Nutr. 2024;6(1):52-56. DOI: 10.33545/26646064.2024.v6.i1a.140