Vol. 7, Issue 12, Part A (2025)

Protein intake and specific amino acids (AAs) roles in the body limited information available, histological studies of rats while consuming green gram were poorly known. Varying germination hours of differently treated green gram protein isolate were introduced in the diets to examine the hepatic histological changes of albino rats. Thirty-five weaned male albino rats were used to determine the histological response in liver, kidney, heart, stomach, small intestine of Albino rats fed with green gram germinated for different hours. There were 7 treatments, with five rats per treatment arranged experimental layout with seven processing methods (Control (casein protein), un-germinated green gram, 12 hr soaked green gram, 12 hr germinated green gram, 24 hr germinated green gram, 36 hr germinated green gram, 48 hr germinated green gram) protein isolate. Histological studies showed that changes in tissue of liver, kidney, heart, stomach, small intestine. The changes observed ranges from mild congestion, cellular infiltration and necrosis of the cell in the liver with methionine deficiency was observed. Methionine in the diet caused histopathological changes in liver, kidneys, heart, stomach and small intestine. This study suggests that protein consumption should be upon recommendations since it has deleterious effects on vital organs, nutrition education program should be carried out to the society for proper consumption of complete protein diet.